Cholesterol is an optically active substance with a known specific rotation of -31.6 °dm-1g-1cm3 in diethyl ether [589 nm and 20°C] . It can be measured in a polarimeter equipped with a standard wavelength of 589 nm and a temperature-controlled tube of 100 mm length. Cholesterol plays an essential role in the viral replication machinery and immune activation . It is also used as part of the lipid adjuvants of mRNA vaccines, among others, and facilitates the entry of the vaccine into the human cell . The Varipol B from SCHMIDT + HAENSCH is the ideal polarimeter for this analysis, which is also required by the US Pharmacopeia . With the integrated CRF-21 Part 11 (electronic signature), it also complies with current FDA regulations.
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